Journal: bioRxiv

Article Title: Multi-omics characterization of SIRT3 metabolism and its adaptation to the presence of amyloid-beta oligomers in nasal epithelial cells

doi: 10.64898/2026.03.13.711520

Figure Lengend Snippet: RT-qPCR and WB of SIRT3 in scramble (SCR) and si SIRT3 conditions (A). Representative images of SCR and si SIRT3 in hNE cell cultures (B). SIRT3 silencing effects on SIRT family mRNA abundance in hNECs (C). RT-qPCR and WB of empty vector (EV) and SIRT3 overexpression (OE) conditions (D). Representative images of EV and SIRT3 OE in hNE cell cultures (E). SIRT3 OE effects on SIRT family mRNA abundance in hNECs (F). Data are presented as mean±SEM . *P<0.05 vs. control group (a.u: arbitrary units).

Article Snippet: Briefly, 1 μg of either SIRT3 (HG13033-UT, Sino Biological) or control GFP (CV026, Sino Biological) plasmid DNA was mixed with Lipofectamine 3000 (Fisher, Waltham, MA, USA, L3000015) at a 1:2 ratio in DMEM and incubated for 15 min at room temperature.

Techniques: Quantitative RT-PCR, Plasmid Preparation, Over Expression, Control

Journal: bioRxiv

Article Title: Multi-omics characterization of SIRT3 metabolism and its adaptation to the presence of amyloid-beta oligomers in nasal epithelial cells

doi: 10.64898/2026.03.13.711520

Figure Lengend Snippet: Overlap between down- and up-regulated DEGs (A) and DPPs (B) from SIRT3 silencing and overexpression conditions. Functional analysis of DEGs, DEPs and DPPs from SIRT3 silencing (si) (C) and overexpression (OE) (D).

Article Snippet: Briefly, 1 μg of either SIRT3 (HG13033-UT, Sino Biological) or control GFP (CV026, Sino Biological) plasmid DNA was mixed with Lipofectamine 3000 (Fisher, Waltham, MA, USA, L3000015) at a 1:2 ratio in DMEM and incubated for 15 min at room temperature.

Techniques: Over Expression, Functional Assay

Journal: bioRxiv

Article Title: Multi-omics characterization of SIRT3 metabolism and its adaptation to the presence of amyloid-beta oligomers in nasal epithelial cells

doi: 10.64898/2026.03.13.711520

Figure Lengend Snippet: Functional mapping of deregulated genes (DEGs), proteins (DEPs) and phosphoproteins (DPPs) upon SIRT3 silencing conditions in hNECs.

Article Snippet: Briefly, 1 μg of either SIRT3 (HG13033-UT, Sino Biological) or control GFP (CV026, Sino Biological) plasmid DNA was mixed with Lipofectamine 3000 (Fisher, Waltham, MA, USA, L3000015) at a 1:2 ratio in DMEM and incubated for 15 min at room temperature.

Techniques: Functional Assay

Journal: bioRxiv

Article Title: Multi-omics characterization of SIRT3 metabolism and its adaptation to the presence of amyloid-beta oligomers in nasal epithelial cells

doi: 10.64898/2026.03.13.711520

Figure Lengend Snippet: Functional mapping of deregulated genes (DEGs), proteins (DEPs) and phosphoproteins (DPPs) upon SIRT3 overexpressing conditions in hNECs.

Article Snippet: Briefly, 1 μg of either SIRT3 (HG13033-UT, Sino Biological) or control GFP (CV026, Sino Biological) plasmid DNA was mixed with Lipofectamine 3000 (Fisher, Waltham, MA, USA, L3000015) at a 1:2 ratio in DMEM and incubated for 15 min at room temperature.

Techniques: Functional Assay

Journal: bioRxiv

Article Title: Multi-omics characterization of SIRT3 metabolism and its adaptation to the presence of amyloid-beta oligomers in nasal epithelial cells

doi: 10.64898/2026.03.13.711520

Figure Lengend Snippet: Steady state protein levels of SIRT 1-3 were measured by Western blotting (WB) at 8-24-48 hours post-treatment (A). * p<0.05 respect to untreated-hNEC control cells. Overlap between down- and up-regulated DPPs from SIRT3 silencing and overexpression upon Aβ oligomer protein stimulation in hNECs (B). Selected differentially phosphorylated proteins affected by SIRT3 modulation in Aβ-treated cells (C). Functional analysis of DPPs from SIRT3 silencing (si) and overexpression (OE) (D).

Article Snippet: Briefly, 1 μg of either SIRT3 (HG13033-UT, Sino Biological) or control GFP (CV026, Sino Biological) plasmid DNA was mixed with Lipofectamine 3000 (Fisher, Waltham, MA, USA, L3000015) at a 1:2 ratio in DMEM and incubated for 15 min at room temperature.

Techniques: Western Blot, Control, Over Expression, Functional Assay

Journal: bioRxiv

Article Title: Multi-omics characterization of SIRT3 metabolism and its adaptation to the presence of amyloid-beta oligomers in nasal epithelial cells

doi: 10.64898/2026.03.13.711520

Figure Lengend Snippet: Predictive activation profile of pathways, biofunctions and upstream regulators at the level of SIRT3 silencing and/or overexpression upon Aβ oligomer considering APP as main hub. Positive z-scores indicate potential activated pathways, whereas negative z-scores refer to predicted inhibited pathways. Based on SIRT3 silencing and overexpression datasets (A). Activation prediction of significantly altered pathways and neuronal functions (B). Systems Biology analysis were performed through the Ingenuity Pathway Analysis software .

Article Snippet: Briefly, 1 μg of either SIRT3 (HG13033-UT, Sino Biological) or control GFP (CV026, Sino Biological) plasmid DNA was mixed with Lipofectamine 3000 (Fisher, Waltham, MA, USA, L3000015) at a 1:2 ratio in DMEM and incubated for 15 min at room temperature.

Techniques: Activation Assay, Over Expression, Software

Journal: International Journal of Surgery (London, England)

Article Title: Targeting SIRT3 to regulate mitophagy-dependent ferroptosis for preventing glucocorticoid-induced osteoporosis

doi: 10.1097/JS9.0000000000002783

Figure Lengend Snippet: SIRT3 suppresses mitophagy-dependent ferroptosis, restoring the osteogenic capacity of MC3T3-E1 cells. (A) Machine learning (LASSO/SVM-RFE/Random Forest) identified GIOP-mitophagy associated genes. (B) SIRT3 expression correlated with GIOP progression. (C) qRT-PCR quantified PINK1/PARKIN mRNA in DEX-treated cells. (D-E) Western blot detected PINK1/PARKIN protein in DEX/DEX + SIRT3 groups. (F) IF visualized SIRT3 alterations (DEX group, 50 μm). (G) Multiplex IF showed GPX4/TFR/PINK1/PARKIN/SIRT3 changes (DEX/DEX + SIRT3, 50 μm). (H-I) Osteogenic capacity assessed by ALP/ARS staining. (J-K) Oxidative stress markers (MDA/GSH) measured. (L) qPCR analyzed PINK1/PARKIN/RUNX2/OPG/SIRT3 expression. Experimental design: n =3 biological replicates (technical triplicates). Data: mean ± SD. Statistics: one-way ANOVA. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).

Article Snippet: A cohort of 40 male Sprague-Dawley rats (8-week-old, SPF-grade) were randomly allocated into four groups (n = 10/group): Control: PBS vehicle Model: GIOP Mdivi-1: GIOP + 15 mg/kg Mdivi-1 (MedChemExpress, HY-15 886) i.p. every 48 h [ ] SIRT3 agonist: GIOP + 200 mg/kg nicotinamide riboside (MedChemExpress, HY-15 452) p.o. daily [ ] .

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Multiplex Assay, Staining

Journal: International Journal of Surgery (London, England)

Article Title: Targeting SIRT3 to regulate mitophagy-dependent ferroptosis for preventing glucocorticoid-induced osteoporosis

doi: 10.1097/JS9.0000000000002783

Figure Lengend Snippet: SIRT3 prevents GIOP by regulating mitophagy-dependent ferroptosis (A) Tibial sections were co-stained for FTH/GPX4 and RUNX2/OPG (scale bar = 500 μm). (B) Intracellular ROS levels were visualized by fluorescence staining (scale bar = 1000 μm). (C) Mitophagy markers PINK1/PARKIN were co-localized by IF (scale bar = 100 μm). (D) Micro-CT reconstructed 2D trabecular architecture. (E) Histomorphometric analysis of bone structure (HE staining, scale bar = 200 μm). Experimental design: n =5 biological replicates/group. Data presented as mean ± SD. Statistical analysis: two-tailed Student’s t-test. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)

Article Snippet: A cohort of 40 male Sprague-Dawley rats (8-week-old, SPF-grade) were randomly allocated into four groups (n = 10/group): Control: PBS vehicle Model: GIOP Mdivi-1: GIOP + 15 mg/kg Mdivi-1 (MedChemExpress, HY-15 886) i.p. every 48 h [ ] SIRT3 agonist: GIOP + 200 mg/kg nicotinamide riboside (MedChemExpress, HY-15 452) p.o. daily [ ] .

Techniques: Staining, Fluorescence, Micro-CT, Two Tailed Test